Structure-Based Development of a Subtype-Selective Orexin 1 Receptor Antagonist

Hellman J, Drabek M, Yin J, Gunera J, Proll T, Kraus F, Langmead CJ, Hubner H, Weikert D, Kolb P, Rosenbaum DM, Gmeiner P. Proceedings of the National Academy of Sciences USA. 117: 18059-18067 (2020).
PDB: 6V9S

Structure of a D2 Dopamine Receptor-G-Protein Complex in a Lipid Membrane

Yin J, Chen KM, Clark MJ, Hijazi M, Kumari P, Bai XC, Sunahara RK, Barth P, Rosenbaum DM. Nature. 584: 125-129 (2020).
PDB: 6VMS
EMDB: 21243, 21244, 21245

Structure of an Allosteric Modulator Bound to the CB1 Cannabinoid Receptor

Shao Z, Yan W, Chapman K, Ramesh K, Ferrell A, Yin J, Wang X, Xu Q, Rosenbaum DM. Nature Chemical Biology. 15: 1199-1205 (2019).
PDB: 6KQI

Improved Strategy for Isoleucine 1H/13C Methyl Labeling in Pichia Pastoris

Ali R, Clark LD, Zahm JA, Lemoff A, Ramesh K, Rosenbaum DM, Rosen MK. Journal of Biomolecular NMR. 73: 687-697 (2019).

An Improved Yeast Surface Display Platform for the Screening of Nanobody Immune Libraries

Uchanski T, Zogg T, Yin J, Yuan D, Wohlkonig A, Fischer B, Rosenbaum DM, Kobilka BK, Pardon E, Steyaert J. Scientific Reports. 9: 382 (2019).

Isotopic Labeling of Eukaryotic Membrane Proteins for NMR Studies of Interactions and Dynamics

Dikiy I, Clark LD, Gardner KH, Rosenbaum DM. Methods in Enzymology. 614: 37-65 (2019).

Crystal Structure of the Human NK1 Tachykinin Receptor

Yin J, Chapman K, Clark LD, Shao Z, Borek D, Xu Q, Wang J, Rosenbaum DM. Proceedings of the National Academy of Sciences USA. 115: 13264-13269 (2018).
PDB: 6E59

On the Use of Pichia Pastoris for Isotopic Labeling of Human GPCRs for NMR Studies

Clark LD, Dikiy I, Rosenbaum DM, Gardner KH. Journal of Biomolecular NMR. 71: 203-211 (2018).

Crystal Structures of Human Orexin 2 Receptor Bound to the Subtype-Selective Antagonist EMPA

Suno R, Kimura KT, Nakane T, Yamashita K, Wang J, Fujiwara T, Yamanaka Y, Im D, Horita S, Tsujimoto H, Tawaramoto MS, Hirokawa T, Nango E, Tono K, Kameshima T, Hatsui T, Joti Y, Yabashi M, Shimamoto K, Yamamotot M, Rosenbaum DM, Iwata S, Shimamura T, Kobayashi T. Structure. 26: 7-19 (2018).
PDB: 5WS3, 5WQC

Ligand Modulation of Sidechain Dynamics in a Wild-Type Human GPCR

Clark LD, Dikiy I, Chapman K, Rodstrom KE, Aramini J, LeVine MV, Khelashvili G, Rasmussen SG, Gardner KH, Rosenbaum DM. Elife. Oct 6; 6: e28505 (2017).

Transporters Revealed

Lee JY, Rosenbaum DM. Cell. 168: 951-953 (2017). 

The Human Orexin/Hypocretin Receptor Crystal Structures

Yin J, Rosenbaum DM. Current Topics in Behavioral Neurosciences. Dec 27, 1-15 (2016)

High-Resolution Crystal Structure of the Human CB1 Cannabinoid Receptor

Shao Z, Yin J, Chapman K, Grzemska M, Clark L, Wang J, Rosenbaum DM. Nature. 540: 602-606 (2016).
PDB: 5U09

Crystal Structure of the Human Sterol Transporter ABCG5/ABCG8

Lee JY, Kinch LN, Borek DM, Wang J, Wang J, Urbatsch IL, Xie XS, Grishin NV, Cohen JC, Otwinowski Z, Hobbs HH, Rosenbaum DM. Nature. 533: 561-564 (2016).

Direct Demonstration That Loop1 of Scap Binds to Loop7: A Crucial Event in Cholesterol Homeostasis

Zhang Y, Lee KM, Kinch LN, Grishin NV, Rosenbaum DM, Brown MS, Goldstein JL, Radhakrishnan A. The Journal of Biological Chemistry. 291: 12888-12896 (2016).

Structure and Ligand-Binding Mechanism of the Human OX1 and OX2 Orexin Receptors

Yin J, Babaoglu K, Brautigam CA, Clark L, Shao Z, Scheuermann TH, Harrell CM, Gotter AL, Roecker AJ, Winrow CJ, Renger JJ, Coleman PJ, Rosenbaum DM. Nature Structural & Molecular Biology. 23: 293-299 (2016).
PDB: 4ZJC, 4ZJ8

Methyl Labeling and TROSY NMR Spectroscopy of Proteins Expressed in the Eukaryote Pichia Pastoris

Clark L, Zahm JA, Ali R, Kukula M, Bian L, Patrie SM, Gardner KH, Rosen MK, Rosenbaum DM. Journal of Biomolecular NMR. 62: 239-245 (2015).

Crystal Structure of the Human OX2 Orexin Receptor Bound to the Insomnia Drug Suvorexant

Yin J, Mobarec JC, Kolb P, Rosenbaum DM. Nature. 519: 247-250 (2015).
PDB: 4S0V

Structure and Dynamics of the M3 Muscarinic Acetylcholine Receptor

Kruse AC, Hu J, Pan AC, Arlow DH, Rosenbaum DM, Rosemond E, Green HF, Liu T, Chae PS, Dror RO, Shaw DE, Weis WI, Wess J, Kobilka BK. Nature. 482: 552-556 (2012).

Structure and Function of an Irreversible Agonist-Beta2 Adrenoceptor Complex

Rosenbaum DM, Zhang C, Lyons JA, Holl R, Aragao D, Arlow DH, Rasmussen SGF, Choi HJ, DeVree BT, Sunahara RK, Chae PS, Gellman SH, Dror RO, Shaw DE, Weis WI, Caffrey M, Gmeiner P, Kobilka BK. Nature. 469: 236-240 (2011).

Structure of a Nanobody-Stabilized Active State of the Beta2 Adrenoceptor

Rasmussen SGF, Choi HJ, Fung JJ, Pardon E, Casarosa P, Chae PS, DeVree BT, Rosenbaum DM, Thian FS, Kobilka TS, Schnapp A, Konetzki I, Sunahara RK, Gellman SH, Pautsch A, Steyaert J, Weis WI, Kobilka BK. Nature. 469: 175-180 (2011).

Ligand-Specific Regulation of the Extracellular Surface of a G-Protein-Coupled Receptor

Bokoch MP, Zou Y, Rasmussen SG, Liu CW, Nygaard R, Rosenbaum DM, Fung JJ, Choi HJ, Thian FS, Kobilka TS, Puglisi JD, Weis WI, Pardo L, Prosser RS, Mueller L, Kobilka BK. Nature. 463: 108-112 (2010).

The Structure and Function of G-Protein-Coupled Receptors

Rosenbaum DM, Rasmussen SGF, Kobilka BK. Nature. 459: 356-363 (2009).

Structure-Based Discovery of Beta2-Adrenergic Receptor Ligands

Kolb P, Rosenbaum DM, Irwin JJ,Fung JJ, Kobilka BK, Shoichet BK. Proceedings of the National Academy of Sciences USA. 106: 6843-6848 (2009).

GPCR Engineering Yields High-Resolution Structural Insights Into Beta2-Adrenergic Receptor Function

Rosenbaum DM, Cherezov V, Hanson MA, Rasmussen SG,Thian FS, Kobilka TS, Choi HJ, Yao XJ, Weis WI, Stevens RC, Kobilka BK. Science. 318: 1266-1273 (2007).

High-Resolution Crystal Structure of an Engineered Human Beta2-Adrenergic G Protein-Coupled Receptor

Cherezov V, Rosenbaum DM, Hanson MA, Rasmussen SG,Thian FS, Kobilka TS, Choi HJ, Kuhn P, Weis WI, Kobilka BK, Stevens RC. Science. 318: 1258-1265 (2007).

Crystal Structure of the Human Beta2 Adrenergic G-Protein-Coupled Receptor

Rasmussen SG, Choi HJ, Rosenbaum DM, Kobilka TS, Thian FS, Edwards PC, Burghammer M, Ratnala VR, Sanishvili R, Fischetti RF, Schertler GF, Weis WI, Kobilka BK. Nature. 450: 383-387 (2007).

Binding and Stability Determinants of the PPAR Gamma Nuclear Receptor-Coactivator Interface as Revealed by Shotgun Alanine Scanning and in Vivo Selection

Phillips KM, Rosenbaum DM, Liu DR. Journal of the American Chemical Society. 128: 11298-11306 (2006).

Efficient and Sequence-Specific DNA-Templated Polymerization of PNA Aldehydes

Rosenbaum DM, Liu DR. Journal of the American Chemical Society. 125: 13924-13925 (2003).

Screening Combinatorial Libraries of de Novo Proteins by Hydrogen−Deuterium Exchange and Electrospray Mass Spectrometry

Rosenbaum DM, Roy S, Hecht MH. Journal of the American Chemical Society. Soc. 121: 9509-9513 (1999).